Coding

Part:BBa_K2976006:Design

Designed by: Jiatong Chen   Group: iGEM19_CPU_CHINA   (2019-08-04)


Anti-PD-L1 peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

GNSTM should be fused to the N terminus of anti-PD-L1 peptide to enhance the stability. The amino acid sequence Asn-Ser-Thr (NST) is a standard N-linked glycosylation sequon, and the amino acids Ger and Met flanking sequon could increase glycosylation frequency in mammals.

Source

Synthetic construct, Chinese patent

References

[1] Jie X.A PD-L1 targeting peptide and its application:CN201810650428.9[P].2018-11-20.

[2] Hung ME, Leonard JN. Stabilization of exosome-targeting peptides via engineered glycosylation. J Biol Chem. 2015;290(13):8166-72